complementation plasmid Search Results


90
Sino Biological c3 orf
C3 Orf, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Addgene inc reverse complement
Reverse Complement, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation reporter and complementation plasmids
Reporter And Complementation Plasmids, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Rocha labs complementation plasmids pnifhkosa
Complementation Plasmids Pnifhkosa, supplied by Rocha labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Federation of European Neuroscience Societies complementation plasmid p415-hfq
Complementation Plasmid P415 Hfq, supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Promega complementation plasmid linearized with apai
Complementation Plasmid Linearized With Apai, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
tiangen biotech co complementation plasmid
Function verification of Ehd5 by genetic <t>complementation</t> and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
Complementation Plasmid, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Pacira Inc complementation plasmid ppacira
Function verification of Ehd5 by genetic <t>complementation</t> and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
Complementation Plasmid Ppacira, supplied by Pacira Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bio Basic Canada ca_c0810/ca_c0811 complement plasmid
Function verification of Ehd5 by genetic <t>complementation</t> and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
Ca C0810/Ca C0811 Complement Plasmid, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
GenScript corporation e. coli complementation plasmids
Function verification of Ehd5 by genetic <t>complementation</t> and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
E. Coli Complementation Plasmids, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Promega plasmids for the split-nanoluciferase complementation system
Function verification of Ehd5 by genetic <t>complementation</t> and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
Plasmids For The Split Nanoluciferase Complementation System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Kirin Brewery Company complementation plasmids
Function verification of Ehd5 by genetic <t>complementation</t> and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
Complementation Plasmids, supplied by Kirin Brewery Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Function verification of Ehd5 by genetic complementation and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.

Journal: The Plant Cell

Article Title: The WD40 domain-containing protein Ehd5 positively regulates flowering in rice ( Oryza sativa )

doi: 10.1093/plcell/koad223

Figure Lengend Snippet: Function verification of Ehd5 by genetic complementation and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.

Article Snippet: To generate a complementation plasmid, an 8,891 bp genomic region including 4,144 bp promoter and coding sequence of Ehd5 was amplified from IL9 with corresponding primers Ehd5-co-F and Ehd5-co-R, and then cloned into plant binary vector pCAMBIA1301 (Cambia) using EasyGeno Assembly Cloning kit (TIANGEN).

Techniques: Over Expression, Transgenic Assay, Comparison, Quantitative RT-PCR, Expressing, Ubiquitin Proteomics, Gene Expression