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Image Search Results
Journal: The Plant Cell
Article Title: The WD40 domain-containing protein Ehd5 positively regulates flowering in rice ( Oryza sativa )
doi: 10.1093/plcell/koad223
Figure Lengend Snippet: Function verification of Ehd5 by genetic complementation and overexpression. A) Flowering phenotypes of 95-d-old IL9 (WT), ehd5 , ehd5 with empty pCAMBIA1301 (p1301/ ehd5 ) and Ehd5 complemented transgenic line ( Ehd5 -CP/ ehd5 ) under NSD conditions. Scale bar = 15 cm. B) Heading date statistics of WT, ehd5 , p1301/ ehd5 , and Ehd5 -CP/ ehd5 under NSD conditions. Statistical analysis was conducted on 30 individuals from each line ( n = 30). Different letters indicate significant differences ( P < 0.05), as determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparison test. C) Flowering phenotypes of 80-d-old WT, ehd5 , Ehd5 -CP/ ehd5 , and Ehd5 overexpression lines ( Ehd5 -OE, Ehd5 -OE/ ehd5 ) under NLD conditions. Scale bars = 5 cm. D) Heading date statistics of 30 individuals of WT, ehd5 , Ehd5 -CP/ ehd5 , Ehd5 -OE, and Ehd5 -OE/ ehd5 under NLD conditions ( n = 30). E) RT-qPCR analysis of Ehd5 expression in WT, Ehd5 -OE/ ehd5 , and ehd5 plants under ASD conditions. The hollow line indicates light conditions, the heavy line indicates dark conditions at the bottom. Each time point represents the mean ± SD of three independent samples. Ubiquitin was used to normalize gene expression.
Article Snippet: To generate a
Techniques: Over Expression, Transgenic Assay, Comparison, Quantitative RT-PCR, Expressing, Ubiquitin Proteomics, Gene Expression